P-140: Protective Effects of Imedeen on Oxidative Stress Induction Spermatogenic Disorders and Fertility Potential of Cyclophosphamide-Treated Male NMRI Mice

Background: Imedeen tablets are revolution in anti-aging skincare. This tablets contains Biomarin complex (rich in natural proteins and Marin polysaccharides) and Lycophence GS complex rich in grape seeds extract and lycophence antioxidant,helps to give the skin protection against both direct and indirect damage that caused by free radicals.Cyclophosphamide as an anticancer agent has been known as a male reproductive toxic agent.Therefore the purpose of this study was to investigate whether Imedeen as an established antioxidant and anti-toxicant might have protective effects on reproductive toxicity of CP by assessment of histological characteristics in male mice or not. Materials and Methods: In this experimental study,60 mature male mice were assigned to six groups,10 mice in each. The animals allocated to control group, CP treated in 12 mg/kg/day, Imedeen treated in a first dosage 111 μg/kg/day,Imedeen treated in second dosage 222 μg/kg/day, CP treated and Imedeen treated in first dosage and the last group CP treated and Imedeen treated in second dosage. After 35 days animals were sacrificed. Histological and histomorphometrical analysis of testis was carried out.serum levels of testosterone was evaluated. Results: In CP treated group the histological and histomorphometrical study of testis confirmed the percentage of seminiferous tubules with positive tubular differentiation (TDI), repopulation (RI) and spermatogenesis (SPI) indexes were significantly (p<0.05) less than those of control and Imedeen treated groups and there was an obvious structural changes such as appearance of vacuolated edema in the interstitial connective tissue between the seminiferous tubules with increased hypertrophic mass of leydig cells;while Imedeen use diminished the adverse effects of CP (p<0.05). Conclusion: The present results highly support the idea that CP can adversely damage the testicular tissue, but Imedeen could effectively prevent these adverse effects by inhibiting of oxidative processes and efficient scavenging of free radicals.